Eppendorf Array Technologies
Silver Quant ® detection system and scanner: Get Gold with Silver
Microarray technologies have gained acceptance in recent years more and more in various application areas. While were realized in the actual microarray platforms, different approaches, in many microarray experiments are still a few staining methods determine the quality of the results. If it is detected using dyes, fluorescent dyes are then often used. Especially with the classical microarray applications, such as gene expression analysis are indispensable especially Cy3-or Cy5-based dye molecules. In the laboratory work occasionally arise even with the fluorescent marker routine application problems, because the dyes are UV-sensitive and need to have in the use of special attention. The stained arrays must be analyzed quickly, since the color is not preserved over a longer period. At high ozone concentrations, the molecules also take damage.
For problems of this kind Eppendorf offers a solution: The Silverquant scanner and detection system. Instead of this fluorescence is colored with the help of silver precipitated and detected.
The detection method is robust, stable and sensitive than conventional fluorescent labels. Another attractive aspect: Since the detection of only a white light source is necessary, are the investment costs for the Silverquant scanner is much lower than for corresponding laser-based devices on the market in this application field.
The implementation and use of the method is very simple: When preparing the test sample to be inserted during the processing of biotin molecules. This will happen in the event of a DNA sample by PCR amplification or reverse transcription with biotinylated primers.For proteins specific biotinylated antibodies. In the second step detects a Nanogold-coupled anti-biotin antibodies, the biotin and thus integrated the corresponding target molecule. The reduction leads to a visible Silberreagenz Precipitating particles of silver to the gold particles. Both the reagents and a coordinated protocol for this color and the scanner that is used for subsequent analysis, have been developed by Eppendorf. Completing the application through a software package to operate the scanner and the raw data analysis of the results. The programs for the operation of the scanner and data analysis are intuitive and allow for easy handling and analysis in a few steps.
The nature of the assays and the target molecules to be analyzed, where the Silverquant detection and scanning system can be used are arbitrary. The only requirement of biotin during the integration process and the assay in a microarray format is based (glass or polymer slides). provides for the multiplex analysis, the Eppendorf, of course Silverquant coordinated protocols are offered.
For more information on Silver Quant ® detection system and scanner as well as other products are under -www.eppendorf biochip.com available.
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