Eppendorf Array Technologies
Parallelized GMO testing
The dual chip ® GMO Microarray provides the Eppendorf ideal screening method for genetically modified organisms in both food and feed and seed in dar. The genetically modified plants have artificially introduced marker designed as herbicide resistance. The proof of this so-modified organisms is accomplished by detecting these markers.
Eppendorf has created through the combination of good reproduction by PCR and multifactorial detection by a microarray-based technology is a sensitive, accurate and wide platform.
Microarrays are miniaturized test platforms, where several individual tests are placed on a solid phase, so that a sample can be analyzed at the same time on different parameters. The detection and binding of parameters to be determined is done by probes (eg DNA or peptides). Once the bond has been the target molecules from the sample to the probe, these can be stained. By comparing the information is placed, at which point any test, we can draw conclusions about the presence of the respective parameters. In this way, can be analyzed a few hundred to several thousand parameters at the same time.
In medical research, microarrays have been known for many years and there are often applied to complex problems, which are influenced by a variety of factors. In food analysis microarrays previously played only a subordinate role, although a possible parallelization of tests in many areas, facilitating work and / or a greater collateral. The field of GMO analysis is one of them, and here was realized with the dual chip CMO currently the only commercially available product.
Today in the COM-Analytics mostly carried out individual tests, the results are evaluated separately to allow a broad view of the sample to be analyzed. This is in line with the number of test marker also a feasible approach, but there are some challenges in itself, when a complex testing is needed. With nearly 200 documented in databases CMO and an unknown number of documented CMO world seems not given this complexity. Although there are a number of known GMO to be met by a few tests, but in order to gain a complete picture and to infer unknown GMO, a technology is needed that easily and quickly query a large number of parameters. The dual chip CMO, this can be achieved: A total of 30 markers allow the identification matrix by a combination of all EU authorized GMOs and enter through the detection of commonly used markers in transgenic plants information on unauthorized and unknown COM. With the current version of the microarray may already have 80% of all known GMO can be detected.
The test itself is part of a system consisting of reagents for the reproduction of the transgenic marker and staining of microarrays, the hardware for processing and analysis of microarrays and a software solution to analyze the received signals. At first glance, such a closed system seems very limiting, but quickly opens up as the only successful strategy when a certain limit to ensure sensitivity at a specified accuracy when users need. That this method is not only a robust and reproducible test, but also ensures comparable sensitivities as the established tests with very high accuracy was demonstrated in a multicenter study. A total of 12 laboratories from 8 countries analyzed using the dual chip CMO and the Silver Quant ® scanning and detection systems with different mixtures of GMOs in different concentrations. The results were from the Joint Research Centre (JRC) of the EU and are statistically analyzed here read.
The Silverquant system and the dual chip CMO allow a broadening of the spectrum with low test of time. This method is a qualitative test that allows the presence of the marker above a certain limit. If, for example in the EU non-authorized GMO demonstrated a qualitative determination is sufficient. If necessary for some of the transgenic plants detected a quantification, then the number of tests can be reduced by the use of the Eppendorf microarray in many cases the number. Eppendorf conducts regular training courses by where the use of this technology can be learned within a day.
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