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bhbhb, Wednesday, June 18, 2014

Food analysis: Fast and Safe pathogen detection by PCR

Andreas Hettich GmbH & Co. KG

Food analysis: Fast and Safe pathogen detection by PCR

Angle rotor 2428 to accommodate
24 micro test tubes with
filter cartridges

To protect the consumer foods are regularly examined for possible harmful ingredients and contamination with toxigenic and pathogenic microorganisms.The microbiological examination of food has traditionally been through the culture of microorganisms in artificial culture media. But long ago, the modern methods of molecular biology have found their way into the food analysis, especially the PCR. By using the PCR (Polymerase Chain Reaction) can be the smallest traces of genetic material evidence and thus determine the microorganisms safely. In addition to the high sensitivity of the PCR method is characterized by its rapidity and by its high specificity.

Tabletop centrifuge MIKRO 200
(without cooling). As MIKRO 200 R
also available with cooling.

The quality of the sample preparation determines the quality of the diagnostic

The sample digestion is an important and crucial for the quality of the outcome process. For the extraction and purification of the pathogen DNA or RNA specific KITS be used in the routine many times. These include the necessary reaction vessels, including special filter inserts and all of the reagents necessary for isolating and purifying DNA or RNA. If the sample digestion carried out with very small volumes, it is called Minipreparations or short preps. For preps laboratory standard 1.5 or 2 ml microliter tubes are used.This has the advantage that the required centrifugation can be carried out in a conventional micro liter centrifuge.

Optimal instrumentation facilitates the daily work in the PCR laboratory

2427-A Angle rotor for holding
30 reaction vessels for
1,5 / 2,0 ml with standard lid

In addition to the thermal cycler, which is necessary for the proper performance of the PCR include microliter centrifuge the basic equipment of a PCR laboratory. They are used to isolate the DNA or RNA from the sample and to perform the necessary cleaning steps before and after the PCR. Since microliter centrifuges can vary greatly in the features and performance is to look for when selecting that the requirements of a PCR laboratories are met by the centrifuge. Are kits for use with spin columns, the required centrifugal acceleration is described in the respective appended protocols. The necessary centrifugal force varies depending on the manufacturer of the kits is a maximum of 20,000 x g

Suitable microliter centrifuge for the PCR laboratory

With a max. Centrifugal acceleration of 21 382 xg, the MIKRO 200 from Hettich is suitable for centrifugation of spin columns from different manufacturers. With its compact design and its performance, these microliter centrifuge has proven many times in the PCR laboratory. It is among the fastest in their class. In addition, the user-friendly this MIKRO 200 facilitates the daily routine. The selection of parameters and the values ​​are entered by means knob quickly and easily. It needs only a short Anzentrifugierens to hurl example, liquid droplets from the rim of the lid down, it is sufficient to press the pulse button. The centrifuge then runs as long as the button is pressed, with the pre-selected speed. Another plus point is its extensive rotor program. For centrifugation of spin columns is a special rotor available. It is constructed so that the covers of the reaction vessels are optimally supported, if centrifuged with open vessels. For high sample throughput is next to the 24-place rotor, a 30-place rotor available. For centrifugation of 8-PCR strips a separate rotor is available. Is used in the preparation of phenol is used, the rotors can be closed with a phenol-resistant cover. This is also equipped with a Biodichtung and can be autoclaved.

Andreas Hettich GmbH & Co. KG
Föhrenstraße 12
D-78532 Tuttlingen Tel: +49 (0) 7461 / 705-0 Fax: +49 (0) 7461 / 705-1125 : Email Inquiry Web: www.hettichlab.com

bhbhb, Wednesday, April 2, 2014

The Power of Thermodynamics ™

Julabo GmbH

The Power of Thermodynamics ™

The new PRESTO ® are a completely new generation of highly dynamic temperature control of Julabo. They are ideal for reactor vessels, material stress tests, or temperature simulations.

Leading technology for modern laboratories

The new PRESTO ® brings Julabo cutting edge technology in modern laboratories. With working temperature ranges from -92 ° C to +250 ° C, high heating and cooling capacities and highly efficient components compensate for these devices exothermic and endothermic reactions extremely fast. Robust and reliable work PRESTO ® units even at elevated ambient temperatures up to +40 ° C. Powerful and maintenance-free pump ensures high flow rates at constant pressure. Viscosity changes of the temperature are dynamically balanced. Through the closed circuit the bath has no contact with the ambient air, which prevents moisture ingress and oxidation.

Maximum ease of use with color touch screen

Especially characteristic of the new PRESTO ® is the integrated 5.7'' color industrial touch screen. He provides a clear and concise information and increases the ease of use. The touchscreen operation by finger tip is simple to do by hand. For the presentation of all important information three views are prepared. A password management for administrator and up to two users simplifies the workflow in the laboratory.

PRESTO ® facilitates the routine laboratory work

The new PRESTO ® work within the working temperature range only with a bath fluid. The filling is easily accessible at the top. A PRESTO ®, you barely hear, thanks to its ultra-quiet operation. PRESTO ® are JULABO and therefore subject to high demands for ease and comfort.So only offer JULABO closed side walls without ventilation slots and without lateral connections. You only need a very small footprint and can laboratory equipment or other PRESTO ® closely placed next to each other.

With extensive interfaces, you have many options, PRESTO ® remote control of devices over networks and to integrate into control systems. The integrated Ethernet interface, you have full access to all operating functions. A USB interface allows data logging and wireless remote control with the proven Julabo WirelessTEMP solution.

Quality "Made in Germany" and comprehensive on-site support

Expert advice, joint search with the customer for appropriate solutions and many other services provide the user JULABO the optimal Temperierlösung. With installation and calibration, provision of documentation for equipment qualification and application training Julabo supports its customers so that they can control their Julabo unit quickly and safely and long-term use.

Julabo GmbH
Eisenbahnstraße 45
D-77960 Seelbach Tel: +49 (0) 7823 / 51-180 Fax: +49 (0) 7823/2491 Email: Inquiry Web: www.julabo.de

bhbhb,

New Ismatec ® peristaltic pump REGLO ICC with four independently controlled channels

Better than ever! The REGLO ICC peristaltic pump

Now with four channels - Conveying, Feeding, starting, stopping, reversing, sucking and calibration - each channel independently controlled by computer or by the control panel on the pump directly.

All these innovations based on the usual precision and accuracy of the traditional peristaltic pumps of Ismatec ®. Only with the ICC REGLO you are able more precise fluidic applications - at different flow rates - perform with only one space-saving pump.

• Each channel independently controlled 
• Increased accuracy through calibration of individual channels 
• Flexibility bi-directional flow direction in each channel 
• Easy USB interface, Windows software, including

A special discount of up to 31 May 2014:

10% to 15% discount on ISMATEC ® REGLO ICC

Select a 2 -, 3 - or 4-channel REGLO ICC and receive a 10% discount on your order. If you order two or more REGLO ICC pumps you get a discount of 15%.

IDEX Health & Science GmbH
Futtererstraße 16
D-97877 Wertheim Tel: +49 (0) 9377 / 9203-0 Fax: +49 (0) 9377/1388 Email: Send Enquiry Web: www.idex-hs.com

bhbhb,

Thermal Field-Flow Fractionation - The innovation in the polymer nanoparticles & Analysis Molar Mass by Thermal FFF coupled with macromolecules

Postnova Analytics

The Postnova TF2000-MALS Series is a newly developed analysis system based on Thermal Field-Flow Fractionation and Multi-Angle Light Scattering. The system is ideal for the separation and characterization of polymers and particles.The separation region extends over an extremely broad molecular weight and particle size range. The separation takes place under mild conditions and without shear. The online coupled and fully integrated into the software Multi-Angle Light Scattering Detector, both the molar mass and particle size can be determined directly, without resorting to standard materials need.

The TF2000-MALS Series is technically similar to a GPC-MALS system set up, but avoiding the usual limitations of SEC / GPC technology. The common in SEC / GPC column is replaced in the TF2000-MALS system by a special separation channel, which contains no stationary phase. Separation and retention take place exclusively under the influence of a thermal separation field. Polymers and particles, such as starch, rubber and latex particles can be up to 10E9, since separated, and from 1 nm to 10 microns readily over a range of 10E4. In contrast to the GPC has the Thermal Field-Flow Fractionation no size exclusion limit, which would limit the separation region for high and ultra high molecular weight. Due to the absence of any stationary phase, there may not be undesirable adsorption effects.

Unique Benefits

• Ultra-high resolution and extremely wide separation region
• No shear forces and gentle separation conditions, particularly for polymers
• Low flow rates result in a reduced solvent consumption
• Reusable channel without consumables, no columns required

The Postnova TF2000-MALS complete system consists of a Thermal Field-Flow Fractionation separation unit and the seamlessly integrated Multi-Angle Light Scattering and refractive index detectors. The unique NovaFFF software platform manages the entire FFF-MALS-RI system, is used for data acquisition and allows the direct calculation of the molecular weight and particle size. Other detectors such as UV, Fluorescence and ELSD may also be coupled.

With the introduction of the new TF2000-MALS-series, has Postnova reached a new milestone in terms of perfecting the thermal FFF-MALS technology, thus making this technology for the first time a wide range of users from the field of polymers, materials science and nanotechnology available. The TF2000-MALS-series is an indispensable tool for every researcher and QA managers, the more complex aspects of the subject, edited and adsorbed high molecular weight polymers and gel materials. The technology offers the particular advantage of a huge separation range combined with high separation efficiency. Polymers, gels and particles can be simultaneously separated and characterized with higher resolution than is usually possible with chromatographic methods. In addition, the typical disadvantages in the use of packed columns can be avoided. These properties make the TF2000-MALS-series to a new standard in the field of polymer and particle characterization.

bhbhb, Saturday, July 10, 2010

ultraCLAVE - unique automation in information technology

MLS

The system ultraCLAVE IV A reduces the manpower in information technology by incomparable ease of use. In addition to the high sample throughput and improved information quality and the safety was significantly increased.

The system ultraCLAVE IV A offers the highest degree of automation in the information technology. The tedious and time-consuming closing and opening individual pressure vessel belongs to the past.The samples are introduced together with the necessary acid mixture in standard quartz or PTFE test tubes and covered with a dust cover.

This will lead to any cross-contamination during the digestion between the individual samples, the reactor before heating with microwave energy with inert gas is charged. The gas pressure causes an extreme increase of the boiling point, whereby the closing of each sample container is unnecessary. In addition, a special micro-filter Blindwerteinschleppungen prevent dust particles and ensures optimum conditions for trace analysis.

With the available sample racks then depending on vessel size up to 90 samples simultaneously open. The sample rack is the pressure of the "ultraCLAVE IV" made in. Without manual intervention, the system is automatically closed. After completion of the decomposition process, the opening is again automatically, and all containers are moved out from the pressure reactor. Through the use of commercial vessels, these can be used directly in the sample changer of analytical instruments further.

All samples are in the same pressure reactor. This absolutely identical conditions are ensured. In each run can reference compounds as internal standards with open-minded. Each individual course will be certified by it. The information can be carried out at 200 bar under the highest conditions of 300 ° C. As a result, an optimal digestion quality results even for difficult samples and high sample weights.

Through the direct microwave heating of the digestion takes place in no time. During the process remains cold, the pressure jacket and is additionally cooled by a circulation thermostat. This causes a rapid cooling after the heating program.

In addition, this particular effect is also positive by an extremely low material stress of the pressure reactor. The wear of sample containers and the pressure reactor is greatly reduced, which results in significant savings in consumables and maintenance costs. All this adds up to less cost analysis for each sample with optimum quality information.

• Microwave digestion system
• Flexible deployment options, many samples simultaneously or difficult samples individually
• 40-90 samples per run
• Highest reproducibility due to automatic descendants of temperature / time curves
• Better information quality and lower blank values
• Fast heating rates with direct microwave energy
• Easy handling and automation reduces personnel
• Certification by internal standards (certification analysis)
• Pressure loads up to 200 bar
• Maximum operating temperature 350 ° C depending on tank material limits
• Lower analysis costs through reduction of consumables
• Less consumables costs
• Highest Safety

bhbhb,

AQS-sheets for water, wastewater and sludge

Erich Schmidt Verlag

AQS-sheets for water, wastewater and sludge

Secured analysis results by
controlled conditions!

Major decisions and actions in the field of environmental protection are based on test results obtained by chemical, physical and biological analysis methods. For assured results investigations under controlled conditions are essential. This applies particularly to

• Sampling and sample preservation
• Sample transport and storage
• Sample preparation and treatment
• Measurement and
• Result evaluation and presentation of results.

For years the acknowledged source of knowledge: AQS

The water analysis requires an ANALYTICAL QUALITY ASSURANCE (AQA), which is a unified basis to implement.The necessary information and work tools can be found in this proven collection of fact sheets on the AQS-frame recommendations.

AQS provides specific job aids:

• Expert information for practitioners working in the laboratory and for anyone dealing in the field of water protection issues with the quality of analysis
• Answers to important questions on analytical quality assurance and statistical quality control
• extensive analysis of technical information on standards to improve the quality of results
• Recommendations and guidelines from the hands of the Federal / State Working Group on Water (LAWA).

AQA: Competence "first hand" - issued by the Federal / Wasser (LAWA)

With AQS you ensure clear and checklistenartige presentations:

• Legal framework
• A purpose (analysis) procedure
• technical and personnel
• necessary preparatory work (eg planning and organization)
• Implementation such as sampling and sample preservation
• possible error
• Documentation, evaluation and reporting of results
• analysis of technical information on standards or other rules of procedure
• Tables, charts, graphs, for example, of procedures, equipment orders, analysis results
• Calculation methods / formulas for determination of analytical values
• Model of checklists and protocols (eg test for algae, Daphnia, luminescent bacteria test and sampling for Schwebstoffproben)

AQS-sheets for water, wastewater and sludge
Looseleaf, 514 pages, € (D) 76, - (including 7% VAT and plus shipping)
approximately one additional delivery per year.
ISBN 978 3503 03 197 9

bhbhb, Saturday, July 3, 2010