Food analysis: Fast and Safe pathogen detection by PCR

Andreas Hettich GmbH & Co. KG

Food analysis: Fast and Safe pathogen detection by PCR

Angle rotor 2428 to accommodate
24 micro test tubes with
filter cartridges

To protect the consumer foods are regularly examined for possible harmful ingredients and contamination with toxigenic and pathogenic microorganisms.The microbiological examination of food has traditionally been through the culture of microorganisms in artificial culture media. But long ago, the modern methods of molecular biology have found their way into the food analysis, especially the PCR. By using the PCR (Polymerase Chain Reaction) can be the smallest traces of genetic material evidence and thus determine the microorganisms safely. In addition to the high sensitivity of the PCR method is characterized by its rapidity and by its high specificity.

Tabletop centrifuge MIKRO 200
(without cooling). As MIKRO 200 R
also available with cooling.

The quality of the sample preparation determines the quality of the diagnostic

The sample digestion is an important and crucial for the quality of the outcome process. For the extraction and purification of the pathogen DNA or RNA specific KITS be used in the routine many times. These include the necessary reaction vessels, including special filter inserts and all of the reagents necessary for isolating and purifying DNA or RNA. If the sample digestion carried out with very small volumes, it is called Minipreparations or short preps. For preps laboratory standard 1.5 or 2 ml microliter tubes are used.This has the advantage that the required centrifugation can be carried out in a conventional micro liter centrifuge.

Optimal instrumentation facilitates the daily work in the PCR laboratory

2427-A Angle rotor for holding
30 reaction vessels for
1,5 / 2,0 ml with standard lid

In addition to the thermal cycler, which is necessary for the proper performance of the PCR include microliter centrifuge the basic equipment of a PCR laboratory. They are used to isolate the DNA or RNA from the sample and to perform the necessary cleaning steps before and after the PCR. Since microliter centrifuges can vary greatly in the features and performance is to look for when selecting that the requirements of a PCR laboratories are met by the centrifuge. Are kits for use with spin columns, the required centrifugal acceleration is described in the respective appended protocols. The necessary centrifugal force varies depending on the manufacturer of the kits is a maximum of 20,000 x g

Suitable microliter centrifuge for the PCR laboratory

With a max. Centrifugal acceleration of 21 382 xg, the MIKRO 200 from Hettich is suitable for centrifugation of spin columns from different manufacturers. With its compact design and its performance, these microliter centrifuge has proven many times in the PCR laboratory. It is among the fastest in their class. In addition, the user-friendly this MIKRO 200 facilitates the daily routine. The selection of parameters and the values ​​are entered by means knob quickly and easily. It needs only a short Anzentrifugierens to hurl example, liquid droplets from the rim of the lid down, it is sufficient to press the pulse button. The centrifuge then runs as long as the button is pressed, with the pre-selected speed. Another plus point is its extensive rotor program. For centrifugation of spin columns is a special rotor available. It is constructed so that the covers of the reaction vessels are optimally supported, if centrifuged with open vessels. For high sample throughput is next to the 24-place rotor, a 30-place rotor available. For centrifugation of 8-PCR strips a separate rotor is available. Is used in the preparation of phenol is used, the rotors can be closed with a phenol-resistant cover. This is also equipped with a Biodichtung and can be autoclaved.

Andreas Hettich GmbH & Co. KG
Föhrenstraße 12
D-78532 Tuttlingen Tel: +49 (0) 7461 / 705-0 Fax: +49 (0) 7461 / 705-1125 : Email Inquiry Web: www.hettichlab.com

bhbhb, Wednesday, April 2, 2014